Intangible assets, especially knowledge and culture management, have been traditionally considered by various companies as valuable resources that can be used to generate a competitive advantage. However, it is unclear how these assets work hand-in-hand – and which type of culture is the most appropriate for the development of knowledge. This study aims to analyze whether or not both flexible and rigid cultures can be implemented by companies in order to promote the development and transfer of knowledge within an organization. A sample of 167 companies from Mexico and Bolivia was obtained, applying a quantitative and correlational investigation. Using Pearson correlation and stepwise regression, it was found that these variables are positive and significant, and that there is a moderate effect of the rigid organizational culture in the relationship between flexible culture and knowledge management. From a holistic perspective, both cultures – flexible and rigid – are necessary as a means to improve the development and transfer of knowledge among employees within an organization.