Abstract
Nanotechnology is expected to open some new aspects to fight and prevent diseases using atomic scale tailoring of materials. The ability to uncover the structure and function of biosystems at the nanoscale, stimulates research leading to improvement in biology, biotechnology, medicine and healthcare. The size of nanomaterials is similar to that of most biological molecules and structures; therefore, nanomaterials can be useful for both in vivo and in vitro biomedical research and applications. The integration of nanomaterials with biology has led to the development of diagnostic devices, contrast agents, analytical tools, physical therapy applications, and drug delivery vehicles . There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy, and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. Nano-silver is used in an increasing number of products. Some of the applications have resulted in the concern of governments and the public, since little is known about the potential risks of nano-silver. In this review, an inventory is made to identify knowledge gaps that have to be filled before risks for both man and the environment can be assessed as reliable as for ‘non-nanosized’ chemicals. It is hypothesized that the toxic effects of nanosilver are due to a combination of the specific properties of silver nanoparticles and the generation of ions from them. The main topic for future research is validation of our ‘0-hypothesis’ that toxic effects of nano-silver are proportional to the activity of free silver ions released by the nanoparticles. Furthermore, it must be determined whether or to what extent nano-silver particles will enter the body. The outcomes of these tests will determine the requirements for further toxicity testing.